Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Primers used for PCR analysis.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Sequencing

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: (a) Expression of GFP in NS1-transfected HEK-293T cells was examined by fluorescence microscopy at (I) 24 hr (II) 48 hr (III) 72 hr posttransfection with 10x magnification and bar size of 25 μ m. (b) The expression of NS1 mRNA in HEK-293 T cells was examined and visualized on 1.5% agarose gel at 100 V for 40 min and stained with GelRed at the indicated times posttransfection. (c) Posttransfection expression levels of NS1 mRNA overtime. Green: after 24 hr, red: after 48 hr, blue: after 72 hr, brown: mock-transfection at 24, 48, and 72 hr posttransfection.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Expressing, Transfection, Fluorescence, Microscopy, Agarose Gel Electrophoresis, Staining

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Percentage of apoptotic cells in mock- and NS1-transfected HEK-293T cells at 24 hr, 48 hr, and 72 hr posttransfection was determined by flow cytometry of cells that stained double-positive for PE Annexin V and 7-AAD.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Transfection, Flow Cytometry, Staining

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Thirteen cytokines' concentration of mock-transfected, B19 NS1-transfected, and untransfected HEK-293T cell are measured at 24, 48, and 72 hr posttransfection. Cytokine concentrations are reported based on a picogram per milliliter as mean. The experiments were carried out two times in duplicate.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Concentration Assay, Transfection

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

doi: 10.3892/ijmm.2020.4502

Figure Lengend Snippet: Identification of the 3′-UTR seed region of SCN2B mRNA targeted by miR-449a. A dual-luciferase activity assay for SCN2B 3′UTR-wt or SCN2B 3′UTR-mut detected firefly and luciferase activities following transfection with (A) miR-449a, (B) miR-9, (C) miR-34a-5p mimic or miR-NC. (A): Relative luciferase activity of miR-449a mimics co-transfected with SCN2B 3′UTR-wt or 3′UTR-mut (left panel) and seed regions of SCN2B gene directly regulated by miR-449a (right panel). NC, negative control; UTR, untranslated region; wt, wild-type; mut, mutant; SCN2B, sodium channel voltage-gated beta 2.

Article Snippet: The artificially cloned sequences were inserted downstream of the luciferase reporter gene, pGL3 (Guangzhou RiboBio Co., Ltd.), to generate the SCN2B 3′-UTR-wt and SCN2B 3′-UTR-mut vectors, as described previously ( , ).

Techniques: Luciferase, Activity Assay, Transfection, Negative Control, Mutagenesis

Journal: BMC Genomics

Article Title: Accurate characterization of the IFITM locus using MiSeq and PacBio sequencing shows genetic variation in Galliformes

doi: 10.1186/s12864-017-3801-8

Figure Lengend Snippet: Locus comparison between PacBio consensus sequence (contig 2) and a portion of chromosome 5 of the two versions of the chicken genome. a : The 203 kb BAC reference sequence contained in the PacBio contig 2 (in the middle) is compared with chromosome 5 of Gallus gallus v4 (top) or v5 (bottom) using ACT, Artemis Comparison Tool. The annotation files for Gallus gallus v4 and PacBio contig 2 have been compressed to allow visualization of the whole BAC; for Gallus gallus v5 it was drawn manually only to visualize location of the locus. b : The chIFITM locus (circled in A) is enlarged in B to show only the chIFITM locus including the flanking genes (this is a 40 kb region extracted from the 203Kb total). Gaps are visible in Gallus gallus v4 represented by white bars (N nucleotides), while these are absent in the comparison with the more complete Gallus gallus v5. The graph does not show differences at the nucleotide level, but only an overall view of the locus. c : Dot Plot comparison graphs of the assembled PacBio contig 2 versus Gallus gallus v5 showing differences not visible when using ACT for the 40Kb region. The region enlarged in the right Dot Plot shows a stretch of the genomic region within the intronic region of the chIFITM3 gene which shows differences with chicken genome assembly v5. d Clustal Omega alignment of the PacBio contig 2 consensus sequences and the chicken genome v5 (portion of the IFITM3 gene corresponding to the gap seen in 2C). In yellow is highlighted the gap

Article Snippet: To complement the new Gallus gallus reference we have focused solely on the chIFITM locus and better elucidated its genetic structure by sequencing a bacterial artificial chromosome (BAC), from the BAC library used to generate the original Gallus gallus genome.

Techniques: Comparison, Sequencing